A chromosome segment substituted line (CSSL) represents an ideal resource for studying quantitative traits like thermotolerance. To develop wheat inter-varietal CSSLs with E6015-3S (a heat-sensitive genotype) being the recipient parent. genome-wide unique DNA markers are urgently needed for marker-assisted selection. In this study. https://www.fitnessgravesyardes.shop/product-category/sensor-base/